Coding

Part:BBa_K2252002:Design

Designed by: Elena Rosca   Group: iGEM17_AshesiGhana   (2017-10-26)


FRET Donor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 46
    Illegal BamHI site found at 734
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The length and type of the spacer had to be considered and research has been done in finding a sequence which was previously used as a successful spacer.


Source

The sequence for this part was retrieved from a research paper along with sequences from IGEM registry for a spacer and golB.

References